- GTX100469 - GeneTex RAD51 antibody

Submitted references Inhibition of checkpoint kinase 1 following gemcitabine-mediated S phase arrest results in CDC7- and CDK2-dependent replication catastrophe.

Warren NJH, Eastman A
The Journal of biological chemistry 2019 Feb 8;294(6):1763-1778

BRCA1 Mutation-Specific Responses to 53BP1 Loss-Induced Homologous Recombination and PARP Inhibitor Resistance.

Nacson J, Krais JJ, Bernhardy AJ, Clausen E, Feng W, Wang Y, Nicolas E, Cai KQ, Tricarico R, Hua X, DiMarcantonio D, Martinez E, Zong D, Handorf EA, Bellacosa A, Testa JR, Nussenzweig A, Gupta GP, Sykes SM, Johnson N
Cell reports 2018 Oct 30;25(5):1384

USP13 regulates the RAP80-BRCA1 complex dependent DNA damage response.

Li Y, Luo K, Yin Y, Wu C, Deng M, Li L, Chen Y, Nowsheen S, Lou Z, Yuan J
Nature communications 2017 Jun 1;8:15752

Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes.

Chakraborty A, Tapryal N, Venkova T, Horikoshi N, Pandita RK, Sarker AH, Sarkar PS, Pandita TK, Hazra TK
Nature communications 2016 Oct 5;7:13049

RECK impedes DNA repair by inhibiting the erbB/JAB1/Rad51 signaling axis and enhances chemosensitivity of breast cancer cells.

Hong KJ, Hsu MC, Hung WC
American journal of cancer research 2015;5(8):2422-30

The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex.

Ge C, Che L, Du C
PloS one 2015;10(12):e0144957

BRUCE regulates DNA double-strand break response by promoting USP8 deubiquitination of BRIT1.

Ge C, Che L, Ren J, Pandita RK, Lu J, Li K, Pandita TK, Du C
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 17;112(11):E1210-9

Mutant IDH1-driven cellular transformation increases RAD51-mediated homologous recombination and temozolomide resistance.

Ohba S, Mukherjee J, See WL, Pieper RO
Cancer research 2014 Sep 1;74(17):4836-44

MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells.

Park JM, Huang S, Tougeron D, Sinicrope FA
PloS one 2013;8(5):e65369

RAD51 mutants cause replication defects and chromosomal instability.

Kim TM, Ko JH, Hu L, Kim SA, Bishop AJ, Vijg J, Montagna C, Hasty P
Molecular and cellular biology 2012 Sep;32(18):3663-80

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
KD Reagent Prod page: KD Reagent Prod page
Antibody Lot Number: 40086

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: H1299 B: HepG2 (GTX27900) 12% SDS PAGE GTX100469 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A:NIH-3T310% SDS PAGE GTX100469 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Rad51 antibody [N1C2] detects Rad51 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rad51 protein stained by Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Rad51 antibody [N1C2] immunoprecipitates Rad51 protein in IP experiments.IP samples: Jurkat whole cell extractA. 40 £gg Jurkat whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of Rad51 protein by 4 £gg Rad51 antibody [N1C2] (GTX100469)5 % SDS-PAGEThe immunoprecipitated Rad51 protein was detected by Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Rad51 antibody [N1C2] detects Rad51 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Rad51 antibody [N1C2] detects Rad51 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.

GTX100469

Antibody data