NB100-148
antibody from Novus Biologicals
Targeting: RAD51
BRCC5, FANCR, HsRad51, HsT16930, RAD51A, RECA
Antibody data
- Antibody Data
- Antigen structure
- References [47]
- Comments [0]
- Validations
- Western blot [7]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- NB100-148 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-148, RRID:AB_10002131
- Product name
- Mouse Monoclonal Rad51 Antibody
- Antibody type
- Monoclonal
- Description
- Antigen Affinity-purified.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Activation of DNA damage repair factors in HPV positive oropharyngeal cancers.
Dibutyl phthalate exposure disrupts the progression of meiotic prophase I by interfering with homologous recombination in fetal mouse oocytes.
Genetic determinants of cellular addiction to DNA polymerase theta.
A Meiotic Checkpoint Alters Repair Partner Bias to Permit Inter-sister Repair of Persistent DSBs.
Assessing effects of germline exposure to environmental toxicants by high-throughput screening in C. elegans.
Novel RNA and DNA strand exchange activity of the PALB2 DNA binding domain and its critical role for DNA repair in cells.
Targeting Phosphopeptide Recognition by the Human BRCA1 Tandem BRCT Domain to Interrupt BRCA1-Dependent Signaling.
Suppression of homology-dependent DNA double-strand break repair induces PARP inhibitor sensitivity in VHL-deficient human renal cell carcinoma.
Krebs-cycle-deficient hereditary cancer syndromes are defined by defects in homologous-recombination DNA repair.
CDC7-dependent transcriptional regulation of RAD54L is essential for tumorigenicity and radio-resistance of glioblastoma.
Polo-like kinase-dependent phosphorylation of the synaptonemal complex protein SYP-4 regulates double-strand break formation through a negative feedback loop.
Cancer-causing mutations in the tumor suppressor PALB2 reveal a novel cancer mechanism using a hidden nuclear export signal in the WD40 repeat motif.
The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites.
Chemopreventive Effects of ROS Targeting in a Murine Model of BRCA1-Deficient Breast Cancer.
Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion.
E4 ligase-specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis.
Nuclear p16INK4a expression predicts enhanced radiation response in head and neck cancers.
Human DNA helicase B functions in cellular homologous recombination and stimulates Rad51-mediated 5'-3' heteroduplex extension in vitro.
Chk1 inhibition in p53-deficient cell lines drives rapid chromosome fragmentation followed by caspase-independent cell death.
Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe.
MutS homologue hMSH5: recombinational DSB repair and non-synonymous polymorphic variants.
Microbeam irradiation of C. elegans nematode in microfluidic channels.
MutS homologue hMSH4: interaction with eIF3f and a role in NHEJ-mediated DSB repair.
DNA damage leads to progressive replicative decline but extends the life span of long-lived mutant animals.
MutS homologue hMSH5: role in cisplatin-induced DNA damage response.
Inhibition of homologous recombination by the PCNA-interacting protein PARI.
The ubiquitin-selective segregase VCP/p97 orchestrates the response to DNA double-strand breaks.
Pairing centers recruit a Polo-like kinase to orchestrate meiotic chromosome dynamics in C. elegans.
A mitotic function for the high-mobility group protein HMG20b regulated by its interaction with the BRC repeats of the BRCA2 tumor suppressor.
ATR and ATM differently regulate WRN to prevent DSBs at stalled replication forks and promote replication fork recovery.
Evidence of an adaptive response targeting DNA nonhomologous end joining and its transmission to bystander cells.
A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks.
DNA polymerase POLN participates in cross-link repair and homologous recombination.
A marker of homologous recombination predicts pathologic complete response to neoadjuvant chemotherapy in primary breast cancer.
NKX3.1 activates cellular response to DNA damage.
DNA-damage response, survival and differentiation in vitro of a human neural stem cell line in relation to ATM expression.
Enhanced radiosensitization of human glioma cells by combining inhibition of poly(ADP-ribose) polymerase with inhibition of heat shock protein 90.
RIDDLE immunodeficiency syndrome is linked to defects in 53BP1-mediated DNA damage signaling.
hXRCC2 enhances ADP/ATP processing and strand exchange by hRAD51.
Phenotypic effects of heterozygosity for a BRCA2 mutation.
Histone deacetylase 4 interacts with 53BP1 to mediate the DNA damage response.
BRCA2-dependent and independent formation of RAD51 nuclear foci.
Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability.
Interactions involving the Rad51 paralogs Rad51C and XRCC3 in human cells.
Interactions involving the Rad51 paralogs Rad51C and XRCC3 in human cells.
Identification and purification of two distinct complexes containing the five RAD51 paralogs.
The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51.
Kono T, Hoover P, Poropatich K, Paunesku T, Mittal BB, Samant S, Laimins LA
Virology 2020 Aug;547:27-34
Virology 2020 Aug;547:27-34
Dibutyl phthalate exposure disrupts the progression of meiotic prophase I by interfering with homologous recombination in fetal mouse oocytes.
Tu Z, Mu X, Chen X, Geng Y, Zhang Y, Li Q, Gao R, Liu T, Wang Y, He J
Environmental pollution (Barking, Essex : 1987) 2019 Sep;252(Pt A):388-398
Environmental pollution (Barking, Essex : 1987) 2019 Sep;252(Pt A):388-398
Genetic determinants of cellular addiction to DNA polymerase theta.
Feng W, Simpson DA, Carvajal-Garcia J, Price BA, Kumar RJ, Mose LE, Wood RD, Rashid N, Purvis JE, Parker JS, Ramsden DA, Gupta GP
Nature communications 2019 Sep 19;10(1):4286
Nature communications 2019 Sep 19;10(1):4286
A Meiotic Checkpoint Alters Repair Partner Bias to Permit Inter-sister Repair of Persistent DSBs.
Garcia-Muse T, Galindo-Diaz U, Garcia-Rubio M, Martin JS, Polanowska J, O'Reilly N, Aguilera A, Boulton SJ
Cell reports 2019 Jan 15;26(3):775-787.e5
Cell reports 2019 Jan 15;26(3):775-787.e5
Assessing effects of germline exposure to environmental toxicants by high-throughput screening in C. elegans.
Shin N, Cuenca L, Karthikraj R, Kannan K, Colaiácovo MP
PLoS genetics 2019 Feb;15(2):e1007975
PLoS genetics 2019 Feb;15(2):e1007975
Novel RNA and DNA strand exchange activity of the PALB2 DNA binding domain and its critical role for DNA repair in cells.
Deveryshetty J, Peterlini T, Ryzhikov M, Brahiti N, Dellaire G, Masson JY, Korolev S
eLife 2019 Apr 29;8
eLife 2019 Apr 29;8
Targeting Phosphopeptide Recognition by the Human BRCA1 Tandem BRCT Domain to Interrupt BRCA1-Dependent Signaling.
Periasamy J, Kurdekar V, Jasti S, Nijaguna MB, Boggaram S, Hurakadli MA, Raina D, Kurup LM, Chintha C, Manjunath K, Goyal A, Sadasivam G, Bharatham K, Padigaru M, Potluri V, Venkitaraman AR
Cell chemical biology 2018 Jun 21;25(6):677-690.e12
Cell chemical biology 2018 Jun 21;25(6):677-690.e12
Suppression of homology-dependent DNA double-strand break repair induces PARP inhibitor sensitivity in VHL-deficient human renal cell carcinoma.
Scanlon SE, Hegan DC, Sulkowski PL, Glazer PM
Oncotarget 2018 Jan 12;9(4):4647-4660
Oncotarget 2018 Jan 12;9(4):4647-4660
Krebs-cycle-deficient hereditary cancer syndromes are defined by defects in homologous-recombination DNA repair.
Sulkowski PL, Sundaram RK, Oeck S, Corso CD, Liu Y, Noorbakhsh S, Niger M, Boeke M, Ueno D, Kalathil AN, Bao X, Li J, Shuch B, Bindra RS, Glazer PM
Nature genetics 2018 Aug;50(8):1086-1092
Nature genetics 2018 Aug;50(8):1086-1092
CDC7-dependent transcriptional regulation of RAD54L is essential for tumorigenicity and radio-resistance of glioblastoma.
Li Q, Xie W, Wang N, Li C, Wang M
Translational oncology 2018 Apr;11(2):300-306
Translational oncology 2018 Apr;11(2):300-306
Polo-like kinase-dependent phosphorylation of the synaptonemal complex protein SYP-4 regulates double-strand break formation through a negative feedback loop.
Nadarajan S, Lambert TJ, Altendorfer E, Gao J, Blower MD, Waters JC, Colaiácovo MP
eLife 2017 Mar 27;6
eLife 2017 Mar 27;6
Cancer-causing mutations in the tumor suppressor PALB2 reveal a novel cancer mechanism using a hidden nuclear export signal in the WD40 repeat motif.
Pauty J, Couturier AM, Rodrigue A, Caron MC, Coulombe Y, Dellaire G, Masson JY
Nucleic acids research 2017 Mar 17;45(5):2644-2657
Nucleic acids research 2017 Mar 17;45(5):2644-2657
The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites.
Ha K, Ma C, Lin H, Tang L, Lian Z, Zhao F, Li JM, Zhen B, Pei H, Han S, Malumbres M, Jin J, Chen H, Zhao Y, Zhu Q, Zhang P
Nature communications 2017 Jun 12;8:15751
Nature communications 2017 Jun 12;8:15751
Chemopreventive Effects of ROS Targeting in a Murine Model of BRCA1-Deficient Breast Cancer.
Li M, Chen Q, Yu X
Cancer research 2017 Jan 15;77(2):448-458
Cancer research 2017 Jan 15;77(2):448-458
Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion.
Phillips AF, Millet AR, Tigano M, Dubois SM, Crimmins H, Babin L, Charpentier M, Piganeau M, Brunet E, Sfeir A
Molecular cell 2017 Feb 2;65(3):527-538.e6
Molecular cell 2017 Feb 2;65(3):527-538.e6
E4 ligase-specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis.
Ackermann L, Schell M, Pokrzywa W, Kevei É, Gartner A, Schumacher B, Hoppe T
Nature structural & molecular biology 2016 Nov;23(11):995-1002
Nature structural & molecular biology 2016 Nov;23(11):995-1002
Nuclear p16INK4a expression predicts enhanced radiation response in head and neck cancers.
Dok R, Abbasi Asbagh L, Van Limbergen EJ, Sablina A, Nuyts S
Oncotarget 2016 Jun 21;7(25):38785-38795
Oncotarget 2016 Jun 21;7(25):38785-38795
Human DNA helicase B functions in cellular homologous recombination and stimulates Rad51-mediated 5'-3' heteroduplex extension in vitro.
Liu H, Yan P, Fanning E
PloS one 2015;10(1):e0116852
PloS one 2015;10(1):e0116852
Chk1 inhibition in p53-deficient cell lines drives rapid chromosome fragmentation followed by caspase-independent cell death.
Del Nagro CJ, Choi J, Xiao Y, Rangell L, Mohan S, Pandita A, Zha J, Jackson PK, O'Brien T
Cell cycle (Georgetown, Tex.) 2014;13(2):303-14
Cell cycle (Georgetown, Tex.) 2014;13(2):303-14
Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe.
Cornago M, Garcia-Alberich C, Blasco-Angulo N, Vall-Llaura N, Nager M, Herreros J, Comella JX, Sanchis D, Llovera M
Cell death & disease 2014 Oct 2;5:e1435
Cell death & disease 2014 Oct 2;5:e1435
MutS homologue hMSH5: recombinational DSB repair and non-synonymous polymorphic variants.
Wu X, Xu Y, Feng K, Tompkins JD, Her C
PloS one 2013;8(9):e73284
PloS one 2013;8(9):e73284
Microbeam irradiation of C. elegans nematode in microfluidic channels.
Buonanno M, Garty G, Grad M, Gendrel M, Hobert O, Brenner DJ
Radiation and environmental biophysics 2013 Nov;52(4):531-7
Radiation and environmental biophysics 2013 Nov;52(4):531-7
MutS homologue hMSH4: interaction with eIF3f and a role in NHEJ-mediated DSB repair.
Chu YL, Wu X, Xu Y, Her C
Molecular cancer 2013 Jun 2;12:51
Molecular cancer 2013 Jun 2;12:51
DNA damage leads to progressive replicative decline but extends the life span of long-lived mutant animals.
Lans H, Lindvall JM, Thijssen K, Karambelas AE, Cupac D, Fensgård O, Jansen G, Hoeijmakers JH, Nilsen H, Vermeulen W
Cell death and differentiation 2013 Dec;20(12):1709-18
Cell death and differentiation 2013 Dec;20(12):1709-18
MutS homologue hMSH5: role in cisplatin-induced DNA damage response.
Tompkins JD, Wu X, Her C
Molecular cancer 2012 Mar 8;11:10
Molecular cancer 2012 Mar 8;11:10
Inhibition of homologous recombination by the PCNA-interacting protein PARI.
Moldovan GL, Dejsuphong D, Petalcorin MI, Hofmann K, Takeda S, Boulton SJ, D'Andrea AD
Molecular cell 2012 Jan 13;45(1):75-86
Molecular cell 2012 Jan 13;45(1):75-86
The ubiquitin-selective segregase VCP/p97 orchestrates the response to DNA double-strand breaks.
Meerang M, Ritz D, Paliwal S, Garajova Z, Bosshard M, Mailand N, Janscak P, Hübscher U, Meyer H, Ramadan K
Nature cell biology 2011 Oct 23;13(11):1376-82
Nature cell biology 2011 Oct 23;13(11):1376-82
Pairing centers recruit a Polo-like kinase to orchestrate meiotic chromosome dynamics in C. elegans.
Harper NC, Rillo R, Jover-Gil S, Assaf ZJ, Bhalla N, Dernburg AF
Developmental cell 2011 Nov 15;21(5):934-47
Developmental cell 2011 Nov 15;21(5):934-47
A mitotic function for the high-mobility group protein HMG20b regulated by its interaction with the BRC repeats of the BRCA2 tumor suppressor.
Lee M, Daniels MJ, Garnett MJ, Venkitaraman AR
Oncogene 2011 Jul 28;30(30):3360-9
Oncogene 2011 Jul 28;30(30):3360-9
ATR and ATM differently regulate WRN to prevent DSBs at stalled replication forks and promote replication fork recovery.
Ammazzalorso F, Pirzio LM, Bignami M, Franchitto A, Pichierri P
The EMBO journal 2010 Sep 15;29(18):3156-69
The EMBO journal 2010 Sep 15;29(18):3156-69
Evidence of an adaptive response targeting DNA nonhomologous end joining and its transmission to bystander cells.
Klammer H, Kadhim M, Iliakis G
Cancer research 2010 Nov 1;70(21):8498-506
Cancer research 2010 Nov 1;70(21):8498-506
A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks.
Suzuki K, Yamauchi M, Oka Y, Suzuki M, Yamashita S
Nucleic acids research 2010 Jul;38(12):e129
Nucleic acids research 2010 Jul;38(12):e129
DNA polymerase POLN participates in cross-link repair and homologous recombination.
Moldovan GL, Madhavan MV, Mirchandani KD, McCaffrey RM, Vinciguerra P, D'Andrea AD
Molecular and cellular biology 2010 Feb;30(4):1088-96
Molecular and cellular biology 2010 Feb;30(4):1088-96
A marker of homologous recombination predicts pathologic complete response to neoadjuvant chemotherapy in primary breast cancer.
Graeser M, McCarthy A, Lord CJ, Savage K, Hills M, Salter J, Orr N, Parton M, Smith IE, Reis-Filho JS, Dowsett M, Ashworth A, Turner NC
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Dec 15;16(24):6159-68
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Dec 15;16(24):6159-68
NKX3.1 activates cellular response to DNA damage.
Bowen C, Gelmann EP
Cancer research 2010 Apr 15;70(8):3089-97
Cancer research 2010 Apr 15;70(8):3089-97
DNA-damage response, survival and differentiation in vitro of a human neural stem cell line in relation to ATM expression.
Carlessi L, De Filippis L, Lecis D, Vescovi A, Delia D
Cell death and differentiation 2009 Jun;16(6):795-806
Cell death and differentiation 2009 Jun;16(6):795-806
Enhanced radiosensitization of human glioma cells by combining inhibition of poly(ADP-ribose) polymerase with inhibition of heat shock protein 90.
Dungey FA, Caldecott KW, Chalmers AJ
Molecular cancer therapeutics 2009 Aug;8(8):2243-54
Molecular cancer therapeutics 2009 Aug;8(8):2243-54
RIDDLE immunodeficiency syndrome is linked to defects in 53BP1-mediated DNA damage signaling.
Stewart GS, Stankovic T, Byrd PJ, Wechsler T, Miller ES, Huissoon A, Drayson MT, West SC, Elledge SJ, Taylor AM
Proceedings of the National Academy of Sciences of the United States of America 2007 Oct 23;104(43):16910-5
Proceedings of the National Academy of Sciences of the United States of America 2007 Oct 23;104(43):16910-5
hXRCC2 enhances ADP/ATP processing and strand exchange by hRAD51.
Shim KS, Schmutte C, Tombline G, Heinen CD, Fishel R
The Journal of biological chemistry 2004 Jul 16;279(29):30385-94
The Journal of biological chemistry 2004 Jul 16;279(29):30385-94
Phenotypic effects of heterozygosity for a BRCA2 mutation.
Warren M, Lord CJ, Masabanda J, Griffin D, Ashworth A
Human molecular genetics 2003 Oct 15;12(20):2645-56
Human molecular genetics 2003 Oct 15;12(20):2645-56
Histone deacetylase 4 interacts with 53BP1 to mediate the DNA damage response.
Kao GD, McKenna WG, Guenther MG, Muschel RJ, Lazar MA, Yen TJ
The Journal of cell biology 2003 Mar 31;160(7):1017-27
The Journal of cell biology 2003 Mar 31;160(7):1017-27
BRCA2-dependent and independent formation of RAD51 nuclear foci.
Tarsounas M, Davies D, West SC
Oncogene 2003 Feb 27;22(8):1115-23
Oncogene 2003 Feb 27;22(8):1115-23
Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability.
French CA, Masson JY, Griffin CS, O'Regan P, West SC, Thacker J
The Journal of biological chemistry 2002 May 31;277(22):19322-30
The Journal of biological chemistry 2002 May 31;277(22):19322-30
Interactions involving the Rad51 paralogs Rad51C and XRCC3 in human cells.
Wiese C, Collins DW, Albala JS, Thompson LH, Kronenberg A, Schild D
Nucleic acids research 2002 Feb 15;30(4):1001-8
Nucleic acids research 2002 Feb 15;30(4):1001-8
Interactions involving the Rad51 paralogs Rad51C and XRCC3 in human cells.
Wiese C, Collins DW, Albala JS, Thompson LH, Kronenberg A, Schild D
Nucleic acids research 2002 Feb 15;30(4):1001-8
Nucleic acids research 2002 Feb 15;30(4):1001-8
Identification and purification of two distinct complexes containing the five RAD51 paralogs.
Masson JY, Tarsounas MC, Stasiak AZ, Stasiak A, Shah R, McIlwraith MJ, Benson FE, West SC
Genes & development 2001 Dec 15;15(24):3296-307
Genes & development 2001 Dec 15;15(24):3296-307
The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51.
Masson JY, Davies AA, Hajibagheri N, Van Dyck E, Benson FE, Stasiak AZ, Stasiak A, West SC
The EMBO journal 1999 Nov 15;18(22):6552-60
The EMBO journal 1999 Nov 15;18(22):6552-60
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad51 Antibody (14B4) [NB100-148] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (70 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Rad51 Antibody (14B4) [NB100-148] - HeLa whole cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40). Rabbit anti-RAD51 antibody was used for subsequent WB detection of immunoprecipated RAD51.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148] - BT483 xenograft. RAD51 antibody 14B4 dilution at 1:200. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15 min.