Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF6784 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Clathrin Heavy Chain 1/CHC17 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Clathrin Heavy Chain 1/CHC17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CHC-22 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q00610- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Clathrin Heavy Chain 1/ CHC17 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and human testis tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Clathrin Heavy Chain 1/CHC17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6784) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Clathrin Heavy Chain 1/CHC17 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Clathrin Heavy Chain 1/CHC17 in HeLa Human Cell Line. Clathrin Heavy Chain 1/CHC17 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Clathrin Heavy Chain 1/CHC17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6784) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Clathrin coated vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
