GTX109000

antibody from GeneTex

Targeting: DKC1 Cbf5, DKC, dyskerin, NAP57, NOLA4, XAP101

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX109000

Provider

GeneTex

Proper citation

GeneTex Cat#GTX109000, RRID:AB_11165396

Product name

DKC1 antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

References

Submitted references

Systematic Discovery of RNA Binding Proteins that Regulate MicroRNA Levels.

Nussbacher JK, Yeo GW
Molecular cell 2018 Mar 15;69(6):1005-1016.e7

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Resources for the Comprehensive Discovery of Functional RNA Elements.

Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13

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RNA helicase DDX21 coordinates transcription and ribosomal RNA processing.

Calo E, Flynn RA, Martin L, Spitale RC, Chang HY, Wysocka J
Nature 2015 Feb 12;518(7538):249-53

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 7.5% SDS PAGE GTX109000 diluted at 1:1000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DKC1(GTX109000) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using DKC1(GTX109000) antibody at 1:250 dilution.