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LearnMA5-34640
antibody from Invitrogen Antibodies
Targeting: DKC1
Cbf5, DKC, dyskerin, NAP57, NOLA4, XAP101
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- MA5-34640 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DKC1 Recombinant Rabbit Monoclonal Antibody (JU34-32)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: A431, rat brain tissue, human kidney tissue, mouse testis tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JU34-32
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DKC1 in A431 cell lysate. Samples were incubated with DKC1 monoclonal antibody (Product # MA5-34640), at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-DKC1 Recombinant Rabbit Monoclonal Antibody (JU34-32) (Product # MA5-34640) and a 57 kDa band corresponding to DKC1 was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), PANC-1 (Lane 2), U-2 OS (Lane 3), HeLa (Lane 4) and HL-60 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescentdetection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of DKC1 was achieved by transfecting Hep G2 with DKC1 specific siRNAs (Silencer® select Product # S4111, 4119). Western blot analysis (Fig. a) was performed using whole cell extracts from the DKC1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with DKC1 Recombinant Rabbit Monoclonal Antibody (JU34-32) (Product # MA5-34640, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to DKC1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DKC1 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with DKC1 monoclonal antibody (Product # MA5-34640), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DKC1 in paraffin-embedded mouse testis tissue. Samples were incubated with DKC1 monoclonal antibody (Product # MA5-34640), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DKC1 in paraffin-embedded rat brain tissue. Samples were incubated with DKC1 monoclonal antibody (Product # MA5-34640).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DKC1 in paraffin-embedded mouse testis tissue. Samples were incubated with DKC1 monoclonal antibody (Product # MA5-34640), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DKC1 in paraffin-embedded human kidney tissue. Samples were incubated with DKC1 monoclonal antibody (Product # MA5-34640), and followed by hematoxylin.
