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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [2]
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Validation data
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- Product number
- ALX-804-221-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-221-C100, RRID:AB_2052560
- Product name
- Ski monoclonal antibody (G8)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Description
- Protein G-affinity purified.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Xenopus
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- G8
- Vial size
- 100 μg
- Storage
- -20°C
Submitted references Heterodimers of the SnoN and Ski oncoproteins form preferentially over homodimers and are more potent transforming agents.
Association of specific DNA binding and transcriptional repression with the transforming and myogenic activities of c-Ski.
Cohen SB, Zheng G, Heyman HC, Stavnezer E
Nucleic acids research 1999 Feb 15;27(4):1006-14
Nucleic acids research 1999 Feb 15;27(4):1006-14
Association of specific DNA binding and transcriptional repression with the transforming and myogenic activities of c-Ski.
Nicol R, Zheng G, Sutrave P, Foster DN, Stavnezer E
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 1999 Apr;10(4):243-54
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 1999 Apr;10(4):243-54
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot: Rat cells infected with (lane 1) pBABEpuro h-Ski expressing full length human Ski proteins of ~95kDa and 100kDa (hyperphosphorylated) and (lane 2) infected with pBABEpuro vector showing endogenous Ski proteins ranging from ~97 kDa to 115 kDa. Blot probed with MAb to Ski (G8) (Prod. No. ALX-804-221) at 1µg/ml.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunocytochemistry: Photomicrograph illustrating retrovirally over-expressed v-Ski infibroblasts stained with MAb to Ski (G8) (Prod. No. ALX-804-221).
