AM09008PU-N

antibody from Acris Antibodies GmbH

Targeting: EPHA2 ECK

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM09008PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM09008PU-N, RRID:AB_1615281

Product name

anti EPHA2 / ECK

Antibody type

Monoclonal

Antigen

Recombinant human EphA2 (559-976 aa) purified from E. coli

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

3D7

Vial size

0.1 ml

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis on mouse brain: The extracts of mouse brain (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EphA2 antibody (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

The extracts of mouse brain (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EphA2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry: Paraffin embedded sections of human colon and gastric cancer tissue were incubated with anti-human EphA2 antibody (1/100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry: Formalin-Fixed, Paraffin-Embedded Human Testis stained with EPHA2 antibody at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry: Formalin-Fixed, Paraffin-Embedded Human Thyroid stained with EPHA2 antibody at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Paraffin embedded sections of human colon and gastric cancer tissue were incubated with anti-human EphA2 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)