APR00490G

antibody from Leading Biology

Targeting: RYR3

Western blot (Recommended by provider)

Antibody Data

Product number

APR00490G

Provider

Leading Biology

Product name

Ryanodine Receptor 3 Antibody

Antibody type

Polyclonal

Description

It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The primary intracellular Ca2+ storage/release organelles in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.The ER and SR contain two Ca2+ release channel families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs).3The Ryanodine receptor family consists of three different isoforms: The skeletal muscleisoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3).3 The Ryanodine receptors are homotetrameric proteins generating a Ca2+ conducting channel. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine (poisonous alkaloid found in the South American plant Ryania speciosa to the Ryanodine receptor causes two major changes in the channel: a reduction in single-channel conductance and a marked increase in the open state probability, leading to anoverall increase/decrease in theCa2+ release capability of the channel.RyR3 is the most widely expressed RyR channel which is predominantly expressed in smooth muscle tissues and certain regions of the brain.Abgent is pleased to offer a highly specific antibody directed against an epitope putatively located at thecytoplasmic regionof the human Ryanodine receptor 3 (RyR3). The Anti-Ryanodine Receptor 3 antibody (#AG1038) can be used in western blot analysis and Immunohistochemical applications. It has been designed to recognize RyR3 from human, rat and mouse samples.

Reactivity

Rat

Host

Rabbit

Storage

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.