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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX79470 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX79470, RRID:AB_11168498
- Product name
- VRK1 antibody [1F6]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of VRK1 in 25 ug of various cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with VRK1 antibody [1F6] at a dilution of 1:750 overnight at 4¢XC on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a HRP-conjugated secondary antibody. Chemiluminescent detection was performed.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of VRK1 (green) in HeLa cells. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (left panel) or with (right panel) VRK1 antibody [1F6] at a dilution of 1:200 overnight at 4¢XC, washed with PBS, and incubated with a proper secondary antibody. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on normal biopsies of deparaffinized human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval, tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and then probed without (left panel) or with (right panel) VRK1 antibody [1F6] at a dilution of 1:200 overnight at 4¢XC in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and Streptavidin-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
