R20186

antibody from NSJ Bioreagents

Targeting: IGHE

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

R20186

Provider

NSJ Bioreagents

Product name

Recombinant Human IgE Antibody

Antibody type

Recombinant

Description

Purified

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

RM122

Vial size

100 µg

Concentration

1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide

Storage

Store the recombinant Human IgE antibody at -20°C.

ELISA

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Sandwich ELISA with human plasma using recombinant Human IgE antibody as the capture (25ng/well) and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ELISA of hIgs shows recombinant Human IgE antibody reacts only to IgEλ from human myeloma and the IgE from human plasma. No cross reactivity with IgG, IgM, IgD, or IgA.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ELISA Titration: the plate was coated with different amounts of hIgE. A serial dilution of recombinant Human IgE antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.