Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB12744 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12744, RRID:AB_10677033
- Product name
- TNNT2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of TNNT2.
- Storage
- Store at 4°C. For long term storage store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Tissue lysate from mouse heart was resolved onto 12% SDS-PAGE and transferred onto NC Membrane, then probed by TNNT2 polyclonal antibody (Cat # PAB12744) at 1 : 500, right lane. Left lane was depleted by immunizing peptide.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human cardiac muscle stained with TNNT2 polyclonal antibody (Cat # PAB12744) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)