Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB1874 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Troponin T (Cardiac) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects purified human Troponin T (Cardiac) in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 200805
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Chemical-defined and albumin-free generation of human atrial and ventricular myocytes from human pluripotent stem cells.
Differentiation and characterization of rhesus monkey atrial and ventricular cardiomyocytes from induced pluripotent stem cells.
Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals.
Stem cells derived from human fetal membranes display multilineage differentiation potential.
Pei F, Jiang J, Bai S, Cao H, Tian L, Zhao Y, Yang C, Dong H, Ma Y
Stem cell research 2017 Mar;19:94-103
Stem cell research 2017 Mar;19:94-103
Differentiation and characterization of rhesus monkey atrial and ventricular cardiomyocytes from induced pluripotent stem cells.
Zhang X, Cao H, Bai S, Huo W, Ma Y
Stem cell research 2017 Apr;20:21-29
Stem cell research 2017 Apr;20:21-29
Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marbán L, Zhang Y, Marbán E
Cardiovascular research 2011 Jan 1;89(1):157-65
Cardiovascular research 2011 Jan 1;89(1):157-65
Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals.
Zhang Q, Jiang J, Han P, Yuan Q, Zhang J, Zhang X, Xu Y, Cao H, Meng Q, Chen L, Tian T, Wang X, Li P, Hescheler J, Ji G, Ma Y
Cell research 2011 Apr;21(4):579-87
Cell research 2011 Apr;21(4):579-87
Stem cells derived from human fetal membranes display multilineage differentiation potential.
Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U
Biology of reproduction 2007 Sep;77(3):577-88
Biology of reproduction 2007 Sep;77(3):577-88
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Troponin T in BG01V Human Embryonic Stem Cells. Troponin T was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Troponin T in Human Heart. Troponin T was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.