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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [2]
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- Product number
- MA1-19076 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD5 Monoclonal Antibody (MEM-32)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T-lymphocytes, most of thymocytes and B-lymphocytes subset (B-1a lymphocytes). This antibody can be used as a capture antibody in a Sandwich ELISA with detection antibody MA1-19438. Western Blot: non-reducing conditions.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MEM-32
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human CD5 using mouse monoclonal antibodies MEM-32, CRIS1, and L17F12 on laurylmaltoside lysates of Jurkat cells and of K562 cells (negative control) under non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-CD5 Monoclonal antibody (Product # MA1-19076) followed by IRDye800-conjugated anti-mouse secondary antibody. CD5 was detected at approximately 62kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD5 (MEM-32) purified Monoclonal antibody (Product # MA1-19076) (concentration in sample 3 µg/mL, GAM APC).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Separation of human CD5 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD5 (MEM-32) purified Monoclonal antibody (Product # MA1-19076) (concentration in sample 3 µg/mL, GAM APC).
