TA501334

antibody from OriGene

Targeting: CD5 LEU1, T1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501334

Provider

OriGene

Proper citation

OriGene Cat#TA501334, RRID:AB_11126906

Product name

Anti-CD5 mouse monoclonal antibody, clone OTI6F7 (formerly 6F7)

Antibody type

Monoclonal

Description

Anti-CD5 mouse monoclonal antibody, clone OTI6F7 (formerly 6F7)

Host

Mouse

Conjugate

Unconjugated

Epitope

CD5

Isotype

IgG

Antibody clone number

OTI6F7

Vial size

100 µl

Concentration

0.96 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD5 monoclonal antibody.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA501334), and then analyzed by flow cytometry.

Validation comment

FC