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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [3]
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- Product number
- MA1-19438 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD5 Monoclonal Antibody (CRIS1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes). This antibody can be used as a detection antibody in a Sandwich ELISA with capture antibody MA1-19076. Western Blot: non-reducing conditions.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CRIS1
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human CD5 using mouse monoclonal antibodies MEM-32, CRIS1, and L17F12 on laurylmaltoside lysates of Jurkat cells and of K562 cells (negative control) under non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/mL of mouse anti-CD5 monoclonal antibody (Product # MA1-19438) followed by IRDye800-conjugated anti-mouse secondary antibody. CD5 was detected at approximately 62 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD5 using a monoclonal antibody (Product # MA1-19438).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD5 using a monoclonal antibody (Product # MA1-19438).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of human peripheral blood lymphocytes (PBL) stained with anti-human CD5 (CRIS1) Monoclonal antibody (Product # MA1-19438) (blue; dilution of purified antibody 1 µg/mL); overlay with mouse IgG2a isotype control (red).
