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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-14560 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD5 Monoclonal Antibody (SP19)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA5-14560 targets CD5 in IHC (P) and shows reactivity with Human samples. The MA5-14560 immunogen is synthetic peptide from the intracellular region of CD5.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SP19
- Vial size
- 500 µL
- Concentration
- 0.003 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Interstitial Mycosis Fungoides With Lichen Sclerosus-Like Clinical and Histopathological Features.
Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital.
The clinical features and histopathologic patterns of folliculotropic mycosis fungoides in a series of 38 cases.
Histopathological, immunophenotypic and clinical particularities and evolution of a case of hepatosplenic T-cell lymphoma in transformation to leukemia.
Analysis of 142 Northern Chinese patients with peripheral T/NK-Cell lymphomas: subtype distribution, clinicopathologic features, and prognosis.
Primary pure squamous cell carcinoma of the thyroid: report and histogenic consideration of a case involving a BRAF mutation.
Mucoepidermoid thymic carcinoma: a challenging mediastinal aspirate.
Secondary involvement of the breast in T-cell non-Hodgkin lymphoma, an unusual example mimicking inflammatory breast carcinoma.
The contribution of bifunctional SkipDewax pretreatment solution, rabbit monoclonal antibodies, and polymer detection systems in immunohistochemistry.
Clinicopathologic and genetic profile of intracranial marginal zone lymphoma: a primary low-grade CNS lymphoma that mimics meningioma.
Tekin B, Kempf W, Seckin D, Ergun T, Yucelten D, Demirkesen C
The American Journal of dermatopathology 2016 Feb;38(2):138-43
The American Journal of dermatopathology 2016 Feb;38(2):138-43
Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital.
Sauter JL, Ambaye AB, Mount SL
Diagnostic cytopathology 2015 Sep;43(9):688-95
Diagnostic cytopathology 2015 Sep;43(9):688-95
The clinical features and histopathologic patterns of folliculotropic mycosis fungoides in a series of 38 cases.
Demirkesen C, Esirgen G, Engin B, Songur A, Oğuz O
Journal of cutaneous pathology 2015 Jan;42(1):22-31
Journal of cutaneous pathology 2015 Jan;42(1):22-31
Histopathological, immunophenotypic and clinical particularities and evolution of a case of hepatosplenic T-cell lymphoma in transformation to leukemia.
Benedek Lázár E, Köpeczi JB, Tunyogi AB, Kakucs E, Horváth E, Turcu M, Benedek I
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2013;54(4):1135-9
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2013;54(4):1135-9
Analysis of 142 Northern Chinese patients with peripheral T/NK-Cell lymphomas: subtype distribution, clinicopathologic features, and prognosis.
Ren YL, Nong L, Zhang S, Zhao J, Zhang XM, Li T
American journal of clinical pathology 2012 Sep;138(3):435-47
American journal of clinical pathology 2012 Sep;138(3):435-47
Primary pure squamous cell carcinoma of the thyroid: report and histogenic consideration of a case involving a BRAF mutation.
Ko YS, Hwang TS, Han HS, Lim SD, Kim WS, Oh SY
Pathology international 2012 Jan;62(1):43-8
Pathology international 2012 Jan;62(1):43-8
Mucoepidermoid thymic carcinoma: a challenging mediastinal aspirate.
Kapila K, Pathan SK, Amir T, Joneja M, Hebbar S, Al-Ayadhy B
Diagnostic cytopathology 2009 Jun;37(6):433-6
Diagnostic cytopathology 2009 Jun;37(6):433-6
Secondary involvement of the breast in T-cell non-Hodgkin lymphoma, an unusual example mimicking inflammatory breast carcinoma.
Kelten C, Kabukcu S, Sen N, Teke Z, Yaren A, Erdem E, Duzcan E
Archives of gynecology and obstetrics 2009 Jul;280(1):149-52
Archives of gynecology and obstetrics 2009 Jul;280(1):149-52
The contribution of bifunctional SkipDewax pretreatment solution, rabbit monoclonal antibodies, and polymer detection systems in immunohistochemistry.
Wong SC, Chan JK, Lo ES, Chan AK, Wong MC, Chan CM, Lam MY, Chan AT
Archives of pathology & laboratory medicine 2007 Jul;131(7):1047-55
Archives of pathology & laboratory medicine 2007 Jul;131(7):1047-55
Clinicopathologic and genetic profile of intracranial marginal zone lymphoma: a primary low-grade CNS lymphoma that mimics meningioma.
Tu PH, Giannini C, Judkins AR, Schwalb JM, Burack R, O'Neill BP, Yachnis AT, Burger PC, Scheithauer BW, Perry A
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2005 Aug 20;23(24):5718-27
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2005 Aug 20;23(24):5718-27
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tonsil stained with CD5 antibody using AEC chromogen. Note memberane staining of T-cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD5 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD5 monoclonal antibody (Product # MA5-14560) at a dilution of 1:2 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
