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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-42901 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Neutrophil elastase Recombinant Rabbit Monoclonal Antibody (8X4E6)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive test controls include: THP-1, U-937.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 8X4E6
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Neutrophil elastase in various cell lines using a Neutrophil elastase Recombinant Monoclonal antibody (Product # MA5-42901) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 3s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Neutrophil elastase in human spleen using a Neutrophil elastase Recombinant Monoclonal antibody (Product # MA5-42901). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Neutrophil elastase in mouse bone marrow using a Recombinant Monoclonal antibody (Product # MA5-42901) at a dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol..
