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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Immunoprecipitation [1]
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- Product number
- GTX130351 - Provider product page
- Provider
- GeneTex
- Product name
- GM130 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references EIF3C-enhanced exosome secretion promotes angiogenesis and tumorigenesis of human hepatocellular carcinoma.
Lee HY, Chen CK, Ho CM, Lee SS, Chang CY, Chen KJ, Jou YS
Oncotarget 2018 Mar 2;9(17):13193-13205
Oncotarget 2018 Mar 2;9(17):13193-13205
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- GM130 antibody detects GM130 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GM130 antibody (GTX130351) diluted by 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GM130 antibody detects GM130 protein at Golgi apparatus and endoplasmic reticulum by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GM130 protein stained by GM130 antibody (GTX130351) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of GM130 protein from HeLa whole cell extracts using 5 £gg of GM130 antibody (GTX130351).Western blot analysis was performed using GM130 antibody (GTX130351).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
