TA501244

antibody from OriGene

Targeting: CYP1A2 CP12, P3-450

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501244

Provider

OriGene

Proper citation

OriGene Cat#TA501244, RRID:AB_11124110

Product name

CYP1A2 (Cytochrome P450 1A2) mouse monoclonal antibody, clone OTI2C6 (formerly 2C6)

Antibody type

Monoclonal

Description

CYP1A2 (Cytochrome P450 1A2) mouse monoclonal antibody, clone OTI2C6 (formerly 2C6)

Host

Mouse

Conjugate

Unconjugated

Epitope

CYP1A2

Isotype

IgG

Antibody clone number

OTI2C6

Vial size

100 µl

Concentration

0.7 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP1A2 (RC221636, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP1A2.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501244)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501244)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-CYP1A2 antibody(TA501244),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC221636 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CYP1A2 antibody(TA501244), and then analyzed by flow cytometry.

Validation comment

FC