PAB29501

antibody from Abnova Corporation

Targeting: APOB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29501

Provider

Abnova Corporation

Product name

APOB polyclonal antibody

Antibody type

Polyclonal

Description

APOB polyclonal antibody raised against recombinant human APOB.

Antigen sequence

NFVASHIANILNSEELDIQDLKKLVKEALKESQLP
TVMDFRKFSRNYQLYKSVSLPSLDPASAKIEGNLI
FDPNNYLPKESMLKTTLTAFGFASADLIE


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of Lane 1: RT-4 cell lysate, Lane 2: U-251 MG, Lane 3: Human plasma, Lane 4: Human Liver tissue, Lane 5: Human Tonsil tissue with APOB polyclonal antibody (Cat# PAB29501) at 1:250 - 1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of Hep G2 cells with APOB polyclonal antibody (Cat# PAB29501) under 1-4 ug/mL working concentration shows positivity in cytoplasm. Antibody staining is shown in green.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human duodenum with APOB polyclonal antibody (Cat# PAB29501) shows distinct positivity in plasma at 1:500 - 1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)