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LearnNB200-314
antibody from Novus Biologicals
Targeting: HNRNPM
CEAR, HNRNPM4, HNRPM, HNRPM4, HTGR1, NAGR1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB200-314 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-314, RRID:AB_10001161
- Product name
- Mouse Monoclonal hnRNP M1-M4 Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified. NB200-314 is specific for all four M proteins of hnRNP (M1, M2, M3 and M4).
- Reactivity
- Human, Rat, Bovine, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.2 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice.
SUMO modification of heterogeneous nuclear ribonucleoproteins.
SUMO modification of heterogeneous nuclear ribonucleoproteins.
The 72/74-kDa polypeptides of the 70-110 S large heterogeneous nuclear ribonucleoprotein complex (LH-nRNP) represent a discrete subset of the hnRNP M protein family.
Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy.
The human hnRNP M proteins: identification of a methionine/arginine-rich repeat motif in ribonucleoproteins.
DNA helicase from mammalian mitochondria.
DNA helicase from mammalian mitochondria.
Llères D, Denegri M, Biggiogera M, Ajuh P, Lamond AI
EMBO reports 2010 Jun;11(6):445-51
EMBO reports 2010 Jun;11(6):445-51
SUMO modification of heterogeneous nuclear ribonucleoproteins.
Vassileva MT, Matunis MJ
Molecular and cellular biology 2004 May;24(9):3623-32
Molecular and cellular biology 2004 May;24(9):3623-32
SUMO modification of heterogeneous nuclear ribonucleoproteins.
Vassileva MT, Matunis MJ
Molecular and cellular biology 2004 May;24(9):3623-32
Molecular and cellular biology 2004 May;24(9):3623-32
The 72/74-kDa polypeptides of the 70-110 S large heterogeneous nuclear ribonucleoprotein complex (LH-nRNP) represent a discrete subset of the hnRNP M protein family.
Kafasla P, Patrinou-Georgoula M, Guialis A
The Biochemical journal 2000 Sep 1;350 Pt 2:495-503
The Biochemical journal 2000 Sep 1;350 Pt 2:495-503
Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy.
Timchenko LT, Miller JW, Timchenko NA, DeVore DR, Datar KV, Lin L, Roberts R, Caskey CT, Swanson MS
Nucleic acids research 1996 Nov 15;24(22):4407-14
Nucleic acids research 1996 Nov 15;24(22):4407-14
The human hnRNP M proteins: identification of a methionine/arginine-rich repeat motif in ribonucleoproteins.
Datar KV, Dreyfuss G, Swanson MS
Nucleic acids research 1993 Feb 11;21(3):439-46
Nucleic acids research 1993 Feb 11;21(3):439-46
DNA helicase from mammalian mitochondria.
Hehman GL, Hauswirth WW
Proceedings of the National Academy of Sciences of the United States of America 1992 Sep 15;89(18):8562-6
Proceedings of the National Academy of Sciences of the United States of America 1992 Sep 15;89(18):8562-6
DNA helicase from mammalian mitochondria.
Hehman GL, Hauswirth WW
Proceedings of the National Academy of Sciences of the United States of America 1992 Sep 15;89(18):8562-6
Proceedings of the National Academy of Sciences of the United States of America 1992 Sep 15;89(18):8562-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hnRNP M1-M4 Antibody (1D8) [NB200-314] - Analysis of hnRNP M1-M4 in HeLa cell lysates using NB200-314.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody (1D8) [NB200-314] - Analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using hnRNP M1-M4 (1D8) antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes with DAB was used for signal development followed by counterstaining with hematoxylin. Nuclear riboprotein staining was observed.
