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- Western blot [3]
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- Product number
- GTX633410 - Provider product page
- Provider
- GeneTex
- Product name
- Glypican-3 antibody [GT2473]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Hepatitis C Virus Mimics Effects of Glypican-3 on CD81 and Promotes Development of Hepatocellular Carcinomas via Activation of Hippo Pathway in Hepatocytes.
Xue Y, Mars WM, Bowen W, Singhi AD, Stoops J, Michalopoulos GK
The American journal of pathology 2018 Jun;188(6):1469-1477
The American journal of pathology 2018 Jun;188(6):1469-1477
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HepG2 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HepG2 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glypican-3 antibody [GT2473] detects Glypican-3 protein at cell membrane by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glypican-3 protein stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:250.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glypican-3 antibody [GT2473] detects Glypican-3 protein at cytoplasm in human ?hepatocellular carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ?hepatocellular carcinoma. Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:100.
