AM06776PU-N

antibody from OriGene

Targeting: GPC3 DGSX, OCI-5, SDYS, SGB, SGBS, SGBS1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM06776PU-N

Provider

OriGene

Product name

Glypican 3 (GPC3) mouse monoclonal antibody, clone 9C2, Purified

Antibody type

Monoclonal

Description

Glypican 3 (GPC3) mouse monoclonal antibody, clone 9C2, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

GPC3

Isotype

IgG

Antibody clone number

9C2

Vial size

100 µg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis using GPC3 antibody against HepG2 (Lane 1), HEK293 (Lane 2), Jurkat (Lane 3), SK-N-SH (Lane 4), PC-12 (Lane 5), F9 (Lane 6)and Mouse liver (Lane 7) cell lysate.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis using GPC3 Lane against Human GPC3 (AA: 55-200) recombinant protein. (Expected MW is 28.5 kDa)

Validation comment

WB

ELISA

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

Validation comment

ELISA

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using GPC3 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using GPC3 antibody with DAB staining.

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GPC3 antibody with DAB staining.

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells using GPC3 antibody (green) and negative control (red).

Validation comment

FC