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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- AM06776PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM06776PU-N, RRID:AB_11217192
- Product name
- anti Glypican-3 / GPC3
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant fragment of Human GPC3 expressed in E. Coli.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9C2
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis using GPC3 antibody Cat.-No AM06776PU-N against HepG2 (Lane 1), HEK293 (Lane 2), Jurkat (Lane 3), SK-N-SH (Lane 4), PC-12 (Lane 5), F9 (Lane 6)and Mouse liver (Lane 7) cell lysate.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis using GPC3 Lane against Human GPC3 (AA: 55-200) recombinant protein. (Expected MW is 28.5 kDa)
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using GPC3 antibody Cat.-No AM06776PU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded liver cancer tissues using GPC3 antibody Cat.-No AM06776PU-N with DAB staining.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GPC3 antibody Cat.-No AM06776PU-N with DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using GPC3 antibody Cat.-No AM06776PU-N (green) and negative control (red).
