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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- MAB2727 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2727, RRID:AB_10629075
- Product name
- NCK2 monoclonal antibody, clone AF3F11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant NCK2.
- Antibody clone number
- AF3F11
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Non-overlapping functions of Nck1 and Nck2 adaptor proteins in T cell activation.
Nck in a complex containing the catalytic subunit of protein phosphatase 1 regulates eukaryotic initiation factor 2alpha signaling and cell survival to endoplasmic reticulum stress.
The phosphotyrosine peptide binding specificity of Nck1 and Nck2 Src homology 2 domains.
The murine Nck SH2/SH3 adaptors are important for the development of mesoderm-derived embryonic structures and for regulating the cellular actin network.
Ngoenkam J, Paensuwan P, Preechanukul K, Khamsri B, Yiemwattana I, Beck-García E, Minguet S, Schamel WW, Pongcharoen S
Cell communication and signaling : CCS 2014 Mar 26;12:21
Cell communication and signaling : CCS 2014 Mar 26;12:21
Nck in a complex containing the catalytic subunit of protein phosphatase 1 regulates eukaryotic initiation factor 2alpha signaling and cell survival to endoplasmic reticulum stress.
Latreille M, Larose L
The Journal of biological chemistry 2006 Sep 8;281(36):26633-44
The Journal of biological chemistry 2006 Sep 8;281(36):26633-44
The phosphotyrosine peptide binding specificity of Nck1 and Nck2 Src homology 2 domains.
Frese S, Schubert WD, Findeis AC, Marquardt T, Roske YS, Stradal TE, Heinz DW
The Journal of biological chemistry 2006 Jun 30;281(26):18236-45
The Journal of biological chemistry 2006 Jun 30;281(26):18236-45
The murine Nck SH2/SH3 adaptors are important for the development of mesoderm-derived embryonic structures and for regulating the cellular actin network.
Bladt F, Aippersbach E, Gelkop S, Strasser GA, Nash P, Tafuri A, Gertler FB, Pawson T
Molecular and cellular biology 2003 Jul;23(13):4586-97
Molecular and cellular biology 2003 Jul;23(13):4586-97
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- Experimental details
- Immunoblot analysis of cell lysates.Lane 1 : U-937 cell lysate. Lnae 2 : HepG2 cell lysate.Lane 3 : A-431 cell lysate.Lane 4 : K-562 cell lysate.Lane 5 : SK-N-MC cell lysate.
