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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF5005 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Cited-2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Cited-2 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Cited2, a transcriptional modulator protein, regulates metabolism in murine embryonic stem cells.
HIF-1α deletion partially rescues defects of hematopoietic stem cell quiescence caused by Cited2 deficiency.
Cited2 gene controls pluripotency and cardiomyocyte differentiation of murine embryonic stem cells through Oct4 gene.
Li Q, Hakimi P, Liu X, Yu WM, Ye F, Fujioka H, Raza S, Shankar E, Tang F, Dunwoodie SL, Danielpour D, Hoppel CL, Ramírez-Bergeron DL, Qu CK, Hanson RW, Yang YC
The Journal of biological chemistry 2014 Jan 3;289(1):251-63
The Journal of biological chemistry 2014 Jan 3;289(1):251-63
HIF-1α deletion partially rescues defects of hematopoietic stem cell quiescence caused by Cited2 deficiency.
Du J, Chen Y, Li Q, Han X, Cheng C, Wang Z, Danielpour D, Dunwoodie SL, Bunting KD, Yang YC
Blood 2012 Mar 22;119(12):2789-98
Blood 2012 Mar 22;119(12):2789-98
Cited2 gene controls pluripotency and cardiomyocyte differentiation of murine embryonic stem cells through Oct4 gene.
Li Q, Ramírez-Bergeron DL, Dunwoodie SL, Yang YC
The Journal of biological chemistry 2012 Aug 17;287(34):29088-100
The Journal of biological chemistry 2012 Aug 17;287(34):29088-100
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human/Mouse Cited-2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cited-2 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Cited-2 by Western Blot. Detection of Human and Mouse Cited-2 by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cited-2 in Human Breast Cancer Tissue. Cited-2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
