AM31147PU-N

antibody from OriGene

Targeting: ATF1 TREB36

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM31147PU-N

Provider

OriGene

Product name

ATF1 mouse monoclonal antibody, clone 3E7, Purified

Antibody type

Monoclonal

Description

ATF1 mouse monoclonal antibody, clone 3E7, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

ATF1

Isotype

IgG

Antibody clone number

3E7

Vial size

50 µg

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot analysis of ATF1 expression in transfected 293T cell line by ATF1 monoclonal antibody, clone 3E7.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of ATF1 over-expressed 293 cell line, cotransfected with ATF1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ATF1 monoclonal antibody (3E7). GAPDH (36.1 kDa) used as specificity and loading control.

Validation comment

WB

ELISA

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ATF1 is 0.3 ng/ml as a capture antibody.

Validation comment

ELISA

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

Validation comment

IHC