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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 11946-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MEDSHKSTT SETAPQPGSA VQGAHISHIA QQVSSLSESE ESQDSSDSIG SSQKAHGILA RRPSYRKILK DLSSEDTRGR KGDGENSGVS AAVTSMSVPT PIYQTSSGQY IAIAPNGALQ LASPGTDGVQ GLQTLTMTNS GSTQQGTTIL QYAQTSDGQQ ILVPSNQVVV QTASGDMQTY QIRTTPSATS LPQTVVMTSP VTLTSQTTKT DDPQLKREIR LMKNREAARE CRRKKKEYVK CLENRVAVLE NQNKTLIEEL KTLKDLYSNK SV (1-271 aa encoded by BC029619)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 11946-1-AP (ATF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 11946-1-AP (ATF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ATF1 (IP:11946-1-AP, 4ug; Detection:11946-1-AP 1:500) with HeLa cells lysate 3200ug.
