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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- NBP2-29949 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal MTCO1 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.4 ml
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MTCO1 Antibody [NBP2-29949] - Western blot analysis in MCF-7 cell line lysates.This demonstrates the COXI antibody detected the COXI protein (arrow)(Kindly provided by Dr. John Wu).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MTCO1 Antibody [NBP2-29949] - 1:2000 dilution Lane 1: human heart lysate Lane 2: mouse spleen lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: MTCO1 Antibody [NBP2-29949] - Analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1/25 dilution, followed by Dylight (R) 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and mitochondrion staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight (R) 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: MTCO1 Antibody [NBP2-29949] - Analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling Pdx1 with at 1/25 dilution, followed by Dylight (R) 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing endoplasmic reticulum staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight (R) 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
