AP9684C

antibody from Abgent

Targeting: MT-CO1 COI, COX1, MTCO1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP9684C

Provider

Abgent

Proper citation

Abgent Cat#AP9684c, RRID:AB_10612667

Product name

COXI Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

COXI Antibody (Center) (Cat. #AP9684c) western blot analysis in MCF-7 cell line lysates.This demonstrates the COXI antibody detected the COXI protein (arrow)(Kindly provided by Dr. John Wu).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of COXI Antibody (Center) (Cat. #AP9684c) in HL-60 cell line lysates (35ug/lane). COXI (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of COXI Antibody (Center)(Cat#AP9684c) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50