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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- R30376 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MTCO1 Antibody
- Antibody type
- Polyclonal
- Antigen
- An amino acid sequence from the C-terminus of human MTCO1 (PYHTFEEPVYMKS) was used as the immunogen for this MTCO1 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the MTCO1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of MTCO1 antibody and Lane 1: SMMC-7721; 2: MCF-7; 3: Raji; 4: SW620 cell lysate
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa mitochondrial enriched fraction (20ug/lane), 2) human HeLa whole cell lysate (20ug/lane), 3) human Caco-2 whole cell lysate (50ug/lane), 4) rat heart whole cell lysate and 5) mouse heart whole cell lysate with MTCO1 antibody. Predicted molecular weight ~57 kDa, also observed at ~40 kDa (ref. 1).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of rat C6 cells
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: MTCO1 antibody testing of human breast cancer tissue
