Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 39-0900 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Parkin Monoclonal Antibody (PRK109)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- PRK109
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Novel monoclonal antibodies demonstrate biochemical variation of brain parkin with age.
Pawlyk AC, Giasson BI, Sampathu DM, Perez FA, Lim KL, Dawson VL, Dawson TM, Palmiter RD, Trojanowski JQ, Lee VM
The Journal of biological chemistry 2003 Nov 28;278(48):48120-8
The Journal of biological chemistry 2003 Nov 28;278(48):48120-8
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain homogenates using Ms anti-Parkin (Product # 39-0900).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Parkin was performed by loading 50 µg of WT (lane 1) and PRKN CRISPR KO (lane 2) SH-SY5Y cell lysates in RIPA buffer onto a 4-15% gradient polyacrylamide gel. Proteins on the blots were visualized with Ponceau staining (below immunoblot). Proteins were transferred to nitrocellulose membrane and blocked in 5% milk for 1 hr. PRKN was detected at approximately 52 kDa (designated by the black arrow) using a PRKN recombinant polyclonal antibody (Product # 39-0900) at a dilution of 1:500 in 5% BSA in TBS with 0.1% Tween 20 (TBST) overnight at 4°C. The peroxidase-conjugated secondary antibody (Product # 62-6520) was diluted to 0.2 µg/mL in TBST with 5% milk for 1 hr. Chemiluminescent detection was performed using Pierce ECL Western Blotting Substrate (Product # 32106). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), SK-N-AS (Lane 2), IMR-32 (Lane 3), A549 (Lane 4), MCF7 (Lane 5), H-1975 (Lane 6), tissue extracts (30 µg lysate) of Mouse brain (Lane 7) and Rat brain (Lane 8). The blot was probed with Anti-Parkin Monoclonal Antibody (Product # 39-0900, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 52 kDa band corresponding to Parkin was observed in brain tissues and its isoform of 31 kDa was observed across the cell lines and tissues tested.
