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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-7291 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-7291, RRID:AB_2279451
- Product name
- Anti-GSK3B
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references PDZRN3 negatively regulates BMP-2-induced osteoblast differentiation through inhibition of Wnt signaling.
Transgenic expression of the amyloid-beta precursor protein-intracellular domain does not induce Alzheimer's Disease-like traits in vivo.
Basal cancer cell survival involves JNK2 suppression of a novel JNK1/c-Jun/Bcl-3 apoptotic network.
Shilianhua extract inhibits GSK-3beta and promotes glucose metabolism.
Maintenance of constitutive IkappaB kinase activity by glycogen synthase kinase-3alpha/beta in pancreatic cancer.
Adipocytes from women with polycystic ovary syndrome demonstrate altered phosphorylation and activity of glycogen synthase kinase 3.
Obesity-associated improvements in metabolic profile through expansion of adipose tissue.
Glycogen synthase kinase 3 alpha and 3 beta have distinct functions during cardiogenesis of zebrafish embryo.
KLHL1/MRP2 mediates neurite outgrowth in a glycogen synthase kinase 3beta-dependent manner.
Regulated intramembrane proteolysis of amyloid precursor protein and regulation of expression of putative target genes.
RNAi-based screening of the human kinome identifies Akt-cooperating kinases: a new approach to designing efficacious multitargeted kinase inhibitors
Human cytomegalovirus major immediate-early proteins and simian virus 40 large T antigen can inhibit apoptosis through activation of the phosphatidylinositide 3'-OH kinase pathway and the cellular kinase Akt.
Honda T, Yamamoto H, Ishii A, Inui M
Molecular biology of the cell 2010 Sep 15;21(18):3269-77
Molecular biology of the cell 2010 Sep 15;21(18):3269-77
Transgenic expression of the amyloid-beta precursor protein-intracellular domain does not induce Alzheimer's Disease-like traits in vivo.
Giliberto L, d'Abramo C, Acker CM, Davies P, D'Adamio L
PloS one 2010 Jul 16;5(7):e11609
PloS one 2010 Jul 16;5(7):e11609
Basal cancer cell survival involves JNK2 suppression of a novel JNK1/c-Jun/Bcl-3 apoptotic network.
Ahmed SU, Milner J
PloS one 2009 Oct 6;4(10):e7305
PloS one 2009 Oct 6;4(10):e7305
Shilianhua extract inhibits GSK-3beta and promotes glucose metabolism.
Yin J, Zuberi A, Gao Z, Liu D, Liu Z, Ye J
American journal of physiology. Endocrinology and metabolism 2009 Jun;296(6):E1275-80
American journal of physiology. Endocrinology and metabolism 2009 Jun;296(6):E1275-80
Maintenance of constitutive IkappaB kinase activity by glycogen synthase kinase-3alpha/beta in pancreatic cancer.
Wilson W 3rd, Baldwin AS
Cancer research 2008 Oct 1;68(19):8156-63
Cancer research 2008 Oct 1;68(19):8156-63
Adipocytes from women with polycystic ovary syndrome demonstrate altered phosphorylation and activity of glycogen synthase kinase 3.
Chang W, Goodarzi MO, Williams H, Magoffin DA, Pall M, Azziz R
Fertility and sterility 2008 Dec;90(6):2291-7
Fertility and sterility 2008 Dec;90(6):2291-7
Obesity-associated improvements in metabolic profile through expansion of adipose tissue.
Kim JY, van de Wall E, Laplante M, Azzara A, Trujillo ME, Hofmann SM, Schraw T, Durand JL, Li H, Li G, Jelicks LA, Mehler MF, Hui DY, Deshaies Y, Shulman GI, Schwartz GJ, Scherer PE
The Journal of clinical investigation 2007 Sep;117(9):2621-37
The Journal of clinical investigation 2007 Sep;117(9):2621-37
Glycogen synthase kinase 3 alpha and 3 beta have distinct functions during cardiogenesis of zebrafish embryo.
Lee HC, Tsai JN, Liao PY, Tsai WY, Lin KY, Chuang CC, Sun CK, Chang WC, Tsai HJ
BMC developmental biology 2007 Aug 3;7:93
BMC developmental biology 2007 Aug 3;7:93
KLHL1/MRP2 mediates neurite outgrowth in a glycogen synthase kinase 3beta-dependent manner.
Seng S, Avraham HK, Jiang S, Venkatesh S, Avraham S
Molecular and cellular biology 2006 Nov;26(22):8371-84
Molecular and cellular biology 2006 Nov;26(22):8371-84
Regulated intramembrane proteolysis of amyloid precursor protein and regulation of expression of putative target genes.
Hébert SS, Serneels L, Tolia A, Craessaerts K, Derks C, Filippov MA, Müller U, De Strooper B
EMBO reports 2006 Jul;7(7):739-45
EMBO reports 2006 Jul;7(7):739-45
RNAi-based screening of the human kinome identifies Akt-cooperating kinases: a new approach to designing efficacious multitargeted kinase inhibitors
S Morgan-Lappe, K W Woods, Q Li, M G Anderson, M E Schurdak, Y Luo, V L Giranda, S W Fesik, J D Leverson
Oncogene 2005 Oct;25(9):1340-1348
Oncogene 2005 Oct;25(9):1340-1348
Human cytomegalovirus major immediate-early proteins and simian virus 40 large T antigen can inhibit apoptosis through activation of the phosphatidylinositide 3'-OH kinase pathway and the cellular kinase Akt.
Yu Y, Alwine JC
Journal of virology 2002 Apr;76(8):3731-8
Journal of virology 2002 Apr;76(8):3731-8
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 48
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human hippocampus shows distinct cytoplasmic and nuclear positivity in neuronal cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
