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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB330R-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCR1/IL-8RA Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human CXCR1/IL-8 RA transfectants but not the parental cell line.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AAA59159- Isotype
- IgG
- Antibody clone number
- 42705R
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCR1/IL-8 RA in Human Stomach Cancer Tissue. CXCR1/IL-8 RA was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330R) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surfaces in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR1/IL-8 RA in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-HumanCXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330R, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL8/IL-8 and Neutralization by Human CXCR1/IL-8 RA Antibody. Recombinant Human CXCL8/IL-8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL8/IL-8 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330R). The ND50 is typically 0.4-2.0 µg/mL.
