AP02276PU-S

antibody from Acris Antibodies GmbH

Targeting: COL7A1 EBD1, EBDCT, EBR1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AP02276PU-S

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP02276PU-S, RRID:AB_1618167

Product name

anti Collagen type VII

Antibody type

Polyclonal

Antigen

Recombinant Murine type VII Collagen (mixture of fragments aa 97-200, aa 479-578, aa 757-967) GST fusion protein­.

Reactivity

Human, Mouse

Host

Rabbit

Vial size

10 µg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Western Blot analysis using AP02276PU Collagen VII antibody.Extract of Murine dermis, recombinant GST-mCol7 (aa 757-967) and GST were separated by SDS-PAGE and Immunoblotted with Immune and Preimmune Serum preadsorbed against GST. IgG from Immune Serum binds cell-derived (arrow) and recombinant fragment (arrow head) of Collagen type VII.Sitaru C et al. (2005). J. Clin. Invest. 115 (4):870-8.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 2. Immunofluorescence of Collagen VII staining in Cryosections of Murine (A) and Human Skin (B). This antibody AP02276PU stains the Dermal-Epidermal Junction (DEJ). Original magnification 250x. Sitaru C et al. (2005). J. Clin. Invest. 115 (4):870-8.