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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 14106-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NAPB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MDNAGKERE AVQLMAEAEK RVKASHSFLR GLFGGNTRIE EACEMYTRAA NMFKMAKNWS AAGNAFCQAA KLHMQLQSKH DSATSFVDAG NAYKKADPQE AINCLNAAID IYTDMGRFTI AAKHHITIAE IYETELVDIE KAIAHYEQSA DYYKGEESNS SANKCLLKVA AYAAQLEQYQ KAIEIYEQVG ANTMDNPLLK YSAKDYFFKA ALCHFIVDEL NAKLALEKYE EMFPAFTDSR ECKLLKKLLE AHEEQNSEAY TEAVKEFDSI SRLDQWLTTM LLRIKKSIQG DGEGDGDLK (1-298 aa encoded by BC060840 )
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.17 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14106-1-AP (NAPB antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14106-1-AP (NAPB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using NAPB antibody 14106-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14106-1-AP ( NAPB antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14106-1-AP ( NAPB antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-NAPB (IP:14106-1-AP, 4ug; Detection:14106-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
