AF5430

antibody from Novus Biologicals

Targeting: CFD ADN, DF, PFD

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

AF5430

Provider

Novus Biologicals

Product name

Sheep Polyclonal Complement Factor D/Adipsin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse Complement Factor D/Adipsin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Complement Factor D is observed and less than 5% cross-reactivity with rhGranzyme A is observed.

Reactivity

Mouse

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina.

Pauly D, Agarwal D, Dana N, Schäfer N, Biber J, Wunderlich KA, Jabri Y, Straub T, Zhang NR, Gautam AK, Weber BHF, Hauck SM, Kim M, Curcio CA, Stambolian D, Li M, Grosche A
Cell reports 2019 Nov 26;29(9):2835-2848.e4

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Contribution of Adipose-Derived Factor D/Adipsin to Complement Alternative Pathway Activation: Lessons from Lipodystrophy.

Wu X, Hutson I, Akk AM, Mascharak S, Pham CTN, Hourcade DE, Brown R, Atkinson JP, Harris CA
Journal of immunology (Baltimore, Md. : 1950) 2018 Apr 15;200(8):2786-2797

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Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina.

Williams JA, Stampoulis D, Gunter CE, Greenwood J, Adamson P, Moss SE
PloS one 2016;11(8):e0161898

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Complement factor B is the downstream effector of TLRs and plays an important role in a mouse model of severe sepsis.

Zou L, Feng Y, Li Y, Zhang M, Chen C, Cai J, Gong Y, Wang L, Thurman JM, Wu X, Atkinson JP, Chao W
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 1;191(11):5625-35

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse Complement Factor D/Adipsin by Western Blot. Western blot shows lysates of mouse plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Complement Factor D/Adipsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5430) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Factor D/Adipsin at approximately 45kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.