AF5430

antibody from R&D Systems

Targeting: CFD ADN, DF, PFD

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

AF5430

Provider

R&D Systems

Product name

Mouse Complement Factor D/Adipsin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse Complement Factor D/Adipsin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Complement Factor D is observed and less than 5% cross-reactivity with rhGranzyme A is observed.

Reactivity

Mouse

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

P03953


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Contribution of Adipose-Derived Factor D/Adipsin to Complement Alternative Pathway Activation: Lessons from Lipodystrophy.

Wu X, Hutson I, Akk AM, Mascharak S, Pham CTN, Hourcade DE, Brown R, Atkinson JP, Harris CA
Journal of immunology (Baltimore, Md. : 1950) 2018 Apr 15;200(8):2786-2797

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Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina.

Williams JA, Stampoulis D, Gunter CE, Greenwood J, Adamson P, Moss SE
PloS one 2016;11(8):e0161898

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Complement factor B is the downstream effector of TLRs and plays an important role in a mouse model of severe sepsis.

Zou L, Feng Y, Li Y, Zhang M, Chen C, Cai J, Gong Y, Wang L, Thurman JM, Wu X, Atkinson JP, Chao W
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 1;191(11):5625-35

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse Complement Factor D/Adipsin by Western Blot. Western blot shows lysates of mouse plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Complement Factor D/Adipsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5430) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Factor D/Adipsin at approximately 45kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.