PAB23617

antibody from Abnova Corporation

Targeting: C11orf49 FLJ22210, MGC4707

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23617

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23617, RRID:AB_11123717

Product name

C11orf49 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant C11orf49.

Antigen sequence

LGALPDKGDLMHDPAMDEELERLLAQVPGLVNSVT
ASPEASCLPSRTPPRVGSPWRPLHHSRKVDGESDG
STEETDESET


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with C11orf49 polyclonal antibody (Cat # PAB23617) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with C11orf49 polyclonal antibody (Cat # PAB23617) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach with C11orf49 polyclonal antibody (Cat # PAB23617) shows strong cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)