sc-511

antibody from Santa Cruz Biotechnology

Targeting: E2F4 E2F-4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-511

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-511, RRID:AB_627484

Product name

Anti-E2F4

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

References

Submitted references

Interaction of YY1 with E2Fs, mediated by RYBP, provides a mechanism for specificity of E2F function

Susanne Schlisio, Terri Halperin, Miguel Vidal, Joseph R. Nevins
The EMBO Journal 2002 Nov;21(21):5775-5786

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Interaction of YY1 with E2Fs, mediated by RYBP, provides a mechanism for specificity of E2F function.

Schlisio S, Halperin T, Vidal M, Nevins JR
The EMBO journal 2002 Nov 1;21(21):5775-86

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 44

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human duodenum shows cytoplasmic and membranous positivity in glandular cells.

Validation comment

No experimental and/or bioinformatic data available, or staining pattern not consistent with experimental and/or bioinformatic data.