Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-B10643 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ RPA2 / RFA2 / RPA34 Antibody LS-B10643
- Antibody type
- Polyclonal
- Description
- Caprylic acid and ammonium sulfate precipitation
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
No comments: Submit comment
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of Replication protein A 32 kDa subunit antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size:32 kDa. Observed band size: 32 kDa Additional bands at: 26 kDa. We are unsure as to the identity of this extra band. This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: 293 whole cell lysate All lanes: RPA2 antibody at 2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30, 31, 39 kDa Observed band size: 30 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating RPA2 in Hela whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of RPA2 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: RPA2 Antibody (6µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating RPA2 in Hela whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of RPA2 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: RPA2 Antibody (6µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-RPA2 / RFA2 / RPA34 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.