100-401-173

antibody from Rockland Immunochemicals, Inc.

Targeting: RBL2 p130, Rb2

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

100-401-173

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#100-401-173, RRID:AB_2175564

Product name

Anti-p130 (Rb2) (RABBIT) Antibody - 100-401-173

Antibody type

Polyclonal

Vial size

100 µl

Immunohistochemistry

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Immunohistochemical staining of mouse tissue using anti-pRb2/p130 antiserum.  The staining shows the location of pRb2/p130 in developing mouse tissue.  Other detection systems should yield similar results. Sections were cut at 5-7 µm, mounted on glass and dried overnight at 37°C.  All sections were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS).  PBS was used for all subsequent washes and for antiserum dilution.  Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum.  Slides were incubated at 20° C for 1 h with rabbit anti-pRb2/p130 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min.  Slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20° C.  Diaminobenzidine was used as the final chromogen.  Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.

Validation comment

Immunohistochemistry