17391-1-AP

antibody from Invitrogen Antibodies

Targeting: KRT6B KRTL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17391-1-AP

Provider

Invitrogen Antibodies

Product name

Cytokeratin 6B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: FEQYINNLR RQLDNIVGER GRLDSELRNM QDLVEDLKNK YEDEINKRTA AENEFVTLKK DVDAAYMNKV ELQAKADTLT DEINFLRALY DAELSQMQTH ISDTSVVLSM DNNRNLDLDS IIAEVKAQYE EIAQRSRAEA ESWYQTKYEE LQITAGRHGD DLRNTKQEIA EINRMIQRLR SEIDHVKKQC ANLQAAIADA EQRGEMALKD AKNKLEGLED ALQKAKQDLA RLLKEYQELM NVKLALDVEI ATYRKLLEGE ECRLNGEGVG QVNISVVQST VSSGYGGASG VGSGLGLGGG SSYSYGSGLG VGGGFSSSSG RATGGGLSSV GGGSSTIKYT TTSSSSRKSY KH (214-564 aa encoded by BC034535)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse skin tissue were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsil using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsil using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human oesophagus using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human oesophagus using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 17391-1-AP ( Cytokeratin 6B antibody at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 17391-1-AP ( Cytokeratin 6B antibody at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were intracellularly stained with 0.2 µg Anti-Human Cytokeratin 6B (Product # 17391-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.2 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Cytokeratin 6B (IP:17391-1-AP, 4ug; Detection:17391-1-AP 1:800) with mouse skin tissue lysate 3200ug.