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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB110-40623 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-40623, RRID:AB_714949
- Product name
- Rabbit Polyclonal HP1 alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Arginine methylation expands the regulatory mechanisms and extends the genomic landscape under E2F control.
Isolation of In Vivo SUMOylated Chromatin-Bound Proteins.
SPOP E3 Ubiquitin Ligase Adaptor Promotes Cellular Senescence by Degrading the SENP7 deSUMOylase.
Differential expression of SUMO-specific protease 7 variants regulates epithelial-mesenchymal transition.
Roworth AP, Carr SM, Liu G, Barczak W, Miller RL, Munro S, Kanapin A, Samsonova A, La Thangue NB
Science advances 2019 Jun;5(6):eaaw4640
Science advances 2019 Jun;5(6):eaaw4640
Isolation of In Vivo SUMOylated Chromatin-Bound Proteins.
Bawa-Khalfe T
Methods in molecular biology (Clifton, N.J.) 2016;1475:205-16
Methods in molecular biology (Clifton, N.J.) 2016;1475:205-16
SPOP E3 Ubiquitin Ligase Adaptor Promotes Cellular Senescence by Degrading the SENP7 deSUMOylase.
Zhu H, Ren S, Bitler BG, Aird KM, Tu Z, Skordalakes E, Zhu Y, Yan J, Sun Y, Zhang R
Cell reports 2015 Nov 10;13(6):1183-1193
Cell reports 2015 Nov 10;13(6):1183-1193
Differential expression of SUMO-specific protease 7 variants regulates epithelial-mesenchymal transition.
Bawa-Khalfe T, Lu LS, Zuo Y, Huang C, Dere R, Lin FM, Yeh ET
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 23;109(43):17466-71
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 23;109(43):17466-71
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HP1 alpha Antibody [NB110-40623] - Whole cell lysate (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB110-40623 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBX5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
