16226-1-AP

antibody from Invitrogen Antibodies

Targeting: PDE4A DPDE2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16226-1-AP

Provider

Invitrogen Antibodies

Product name

PDE4A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MVIDMVLAT DMSKHMTLLA DLKTMVETKK VTSSGVLLLD NYSDRIQVLR NMVHCADLSN PTKPLELYRQ WTDRIMAEFF QQGDRERERG MEISPMCDKH TASVEKSQVG FIDYIVHPLW ETWADLVHPD AQEILDTLED NRDWYYSAIR QSPSPPPEEE SRGPGHPPLP DKFQFELTLE EEEEEEISMA QIPCTAQEAL TAQGLSGVEE ALDATIAWEA SPAQESLEVM AQEASLEAEL EAVYLTQQAQ STGSAPVAPD EFSSREEFVV AVSHSSPSAL ALQSPLLPAW RTLSVSEHAP GLPGLPSTAA EVEAQREHQA AKRACSACAG TFGEDTSALP APGGGGSGGD PT (297-647 aa encoded by BC019864)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 16226-1-AP (PDE4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 16226-1-AP (PDE4A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 16226-1-AP (PDE4A antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 16226-1-AP (PDE4A antibody) at dilution of 1:50 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 SH-SY5Y cells were stained with 0.2ug PDE4A antibody (16226-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PDE4A (IP:16226-1-AP, 4ug; Detection:16226-1-AP 1:300) with mouse brain tissue lysate 4000ug.