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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NBP2-22527 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal RAP1A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Investigating the Role of G Protein βγ in Kv7-Dependent Relaxations of the Rat Vasculature.
Stott JB, Barrese V, Suresh M, Masoodi S, Greenwood IA
Arteriosclerosis, thrombosis, and vascular biology 2018 Sep;38(9):2091-2102
Arteriosclerosis, thrombosis, and vascular biology 2018 Sep;38(9):2091-2102
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 whole cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample BUffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human colon carcinoma (right) compared to a negative control without primary antibody (left).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human kidney tissue (right) compared to a negative control without primary antibody (left).
