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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- AF3767 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Rap1A/B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse and rat Rap1A/B. The antibody is highly specific for Rap1A/B and detects recombinant human Rap2A only upon prolonged film exposure.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P62834- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat Rap1A/B by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MDA-MB-468 human breast cancer cell line, PT18 mouse mast/basophil cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat Rap1A/B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Rap1A/B was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Rap1A/B by Western Blot. Western blot shows recombinant human Rap1A, Rap1B, Rap2A, and Rap2B (1 ng/lane). PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat Rap1A/B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
