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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- UM500065 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM500065, RRID:AB_2629078
- Product name
- ERG mouse monoclonal antibody,clone UMAB77
- Antibody type
- Monoclonal
- Description
- ERG mouse monoclonal antibody,clone UMAB77
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ERG
- Isotype
- IgG
- Antibody clone number
- UMAB77
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of cell lysates (35ug) from 8 different cell lines (1: HeLa, 2: SV-T2, 3: A549. 4: COS7, 5: Jurkat, 6: MDCK, 7: PC-12, 8: MCF7).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-ERG mouse monoclonal antibody (UM500065, green, 1:50). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil using anti-ERG mouse monoclonal antibody. (UM500065; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IHC staining of paraffin-embedded carcinoma of human prostate using anti-ERG clone UMAB77 at 1:200 dilution of 0.8mg/mL and detection with Polink2 Broad HRP DAB. UM500056 requires heat-induced epitope retrieval with EDTA (pH8.7) at 95-100C 20 min. The composit image shows 3 prostate tumors with no staining in tumor cells of figure A and B although strong nuclear staining is present in the endothelial cells. Figure C shows strong nuclear with weak cytoplasmic staining in prostate tumor cells.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERG mouse monoclonal antibody (UM500065). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
- Validation comment
- 10K-CHIP
