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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-961 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-961, RRID:AB_631927
- Product name
- Anti-MAP2K3
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references MEK2 is sufficient but not necessary for proliferation and anchorage-independent growth of SK-MEL-28 melanoma cells.
Capillary morphogenesis protein-2 is the major receptor mediating lethality of anthrax toxin in vivo.
Matrix metalloproteinase-activated anthrax lethal toxin demonstrates high potency in targeting tumor vasculature.
Bcl-2 family and the central nervous system: from rheostat to real complex
Relatedness of archaebacterial RNA polymerase core subunits to their eubacterial and eukaryotic equivalents.
Lee CS, Dykema KJ, Hawkins DM, Cherba DM, Webb CP, Furge KA, Duesbery NS
PloS one 2011 Feb 18;6(2):e17165
PloS one 2011 Feb 18;6(2):e17165
Capillary morphogenesis protein-2 is the major receptor mediating lethality of anthrax toxin in vivo.
Liu S, Crown D, Miller-Randolph S, Moayeri M, Wang H, Hu H, Morley T, Leppla SH
Proceedings of the National Academy of Sciences of the United States of America 2009 Jul 28;106(30):12424-9
Proceedings of the National Academy of Sciences of the United States of America 2009 Jul 28;106(30):12424-9
Matrix metalloproteinase-activated anthrax lethal toxin demonstrates high potency in targeting tumor vasculature.
Liu S, Wang H, Currie BM, Molinolo A, Leung HJ, Moayeri M, Basile JR, Alfano RW, Gutkind JS, Frankel AE, Bugge TH, Leppla SH
The Journal of biological chemistry 2008 Jan 4;283(1):529-540
The Journal of biological chemistry 2008 Jan 4;283(1):529-540
Bcl-2 family and the central nervous system: from rheostat to real complex
J J Shacka, K A Roth
Cell Death and Differentiation 2006 May;13(8):1299-1304
Cell Death and Differentiation 2006 May;13(8):1299-1304
Relatedness of archaebacterial RNA polymerase core subunits to their eubacterial and eukaryotic equivalents.
Berghöfer B, Kröckel L, Körtner C, Truss M, Schallenberg J, Klein A
Nucleic acids research 1988 Aug 25;16(16):8113-28
Nucleic acids research 1988 Aug 25;16(16):8113-28
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 36
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human skeletal muscle shows cytoplasmic positivity in myocytes.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
