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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00005606-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005606-M02, RRID:AB_540408
- Product name
- Mouse Monoclonal MKK3/MEK3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MAP2K3 - mitogen-activated protein kinase kinase 3
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Antibody-based proteomics for esophageal cancer: Identification of proteins in the nuclear factor-kappaB pathway and mitotic checkpoint.
Uemura N, Nakanishi Y, Kato H, Nagino M, Hirohashi S, Kondo T
Cancer science 2009 Sep;100(9):1612-22
Cancer science 2009 Sep;100(9):1612-22
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Western Blot analysis of MAP2K3 expression in Hela NE ( Cat # L013V3 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of MAP2K3 expression in transfected 293T cell line by MAP2K3 monoclonal antibody (M02), clone 1D10.Lane 1: MAP2K3 transfected lysate (Predicted MW: 39.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Detection limit for recombinant GST tagged MAP2K3 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of MAP2K3 transfected lysate using anti-MAP2K3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAP2K3 monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of monoclonal antibody to MAP2K3 on formalin-fixed paraffin-embedded human dysgerminoma. Antibody concentration 3 ug/ml.
